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Immunohistochemistry of glial reaction after injury in the rat: Double stainings and markers of cell proliferation
Author(s) -
Schiffer D.,
Giordana M.T.,
Cavalla P.,
Vigliani M.C.,
Attanasio A.
Publication year - 1993
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(93)90085-r
Subject(s) - vimentin , glial fibrillary acidic protein , pathology , proliferating cell nuclear antigen , immunohistochemistry , lesion , proliferation marker , astrocyte , glial scar , biology , hyperplasia , bromodeoxyuridine , intermediate filament , neuroglia , cell , medicine , central nervous system , endocrinology , cytoskeleton , genetics
The astrocytic reaction in the rat after brain injury has been studied immunohistochemically for intermediate filaments (GFAP and vimentin), also with double staining procedures, and for markers of proliferation (BrdU and PCNA). GFAP‐positive reactive astrocytes appeared around the lesion, where they were vimentin‐positive and at a distance. BrdU and PCNA showed a high labelling index around the wound at day 2 and scattered positive nuclei were also found at a distance in the ipsilateral side. BrdU‐positive astrocytes represented a minor fraction of GFAP‐ and vimentin‐positive astrocytes. The expression of vimentin persisted at least 15 days after the lesion. Our results could suggest that distant reactive astrocytes originate through hypertrophy while those close to lesion arise by hyperplasia from mature or immature glial cells. The hypothesis is formulated that cells of the periventricular matrix contribute to the post‐traumatic proliferative activity.