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Effect of CDP‐choline treatment on mitochondrial and synaptosomal protein composition in different brain regions during aging
Author(s) -
Villa Roberto F.,
Ingrao Francesca,
Magri Grazia,
Gorini Antonella,
Reale Salvatore,
Costa Antonino,
Ragusa Nicolo',
Avola Roberto,
GiuffridaStella Anna Maria
Publication year - 1993
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(93)90037-e
Subject(s) - choline , mitochondrion , nucleotide , inner mitochondrial membrane , biology , endocrinology , medicine , metabolism , biochemistry , chemistry , gene
Several age‐dependent modifications of inner mitochondrial membrane and synaptosomal plasma membrane proteins from different brain regions of 4‐, 12‐, 18‐ and 24‐month‐old male Wistar rats, were observed. Some proteins, identified by immunoblotting assay as various subunits of mitochondrial respiratory chain complexes and calmodulin, were particularly impaired. Chronic treatment with CDP‐choline at a dose of 20 mg/kg body weight per day for 28 days caused significant changes in the amounts of several of the above mentioned proteins. Most of the proteins, which decreased during aging, showed a significant increase after CDP‐choline treatment compared with the corresponding control values at the same age. The effect of CDP‐choline might be due to: — the increased availability of cytidylic nucleotides, which in the brain are present in limited amounts compared to the other nucleotides; — the increased content of total adenine nucleotides; —the improvement of brain energy metabolism.