Premium
Effect of the substrate on neurofilament phosphorylation in mixed cultures of rat embryo spinal cord and dorsal root ganglia
Author(s) -
Dahl D.,
Gilad V.H.,
Maggini L.,
Bignami A.
Publication year - 1992
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(92)90039-3
Subject(s) - neurofilament , spinal cord , neurite , fibronectin , laminin , polylysine , microbiology and biotechnology , embryo , phosphorylation , biology , xenopus , anatomy , neuroscience , immunology , immunohistochemistry , in vitro , extracellular matrix , biochemistry , gene
The effect of the substrate on neurofilament phosphorylation was studied in primary cultures of spinal cord and dorsal root ganglia dissociated from 15‐day‐old rat embryos. On polylysine and Primaria® substrates, spinal cord neurons formed aggregates connected by bundles of neurites. (Primaria® dishes have a modified plastic surface with a net positive charge.) On both polylysine and Primaria® substrates, spinal cord neurons were stained with neurofilament monoclonal antibodies reacting with phosphorylated epitopes appearing early in rat embryo development, i.e. soon after neurofilament expression. Conversely, immunoreactivity with antibodies recognizing late phosphorylation events was only observed on Primaria® substrates. As reported by many investigators, fibronectin and laminin were excellent substrates for dorsal root ganglia neurons in culture. However, on both laminin and fibronectin substrates immunoreactivity with antibodies recognizing late phosphorylation events, only occurred after several days in culture, at a time when non‐neuronal cells (mainly astrocytes) had formed a confluent monolayer.