Premium
Effect of seizure activity and calpain inhibitor I on LTP in juvenile hippocampal slices
Author(s) -
Fitzpatrick John S.,
Shahi Kavian,
Baudry Michel
Publication year - 1992
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(92)90020-z
Subject(s) - long term potentiation , hippocampal formation , kainic acid , kainate receptor , juvenile , stimulation , calpain , neuroscience , biology , nmda receptor , medicine , glutamate receptor , ampa receptor , biochemistry , receptor , genetics , enzyme
Kainic acid‐induced seizure activity in adult rats produces an impairment of long‐term potentiation induction in hippocampal slices. As the consequences of seizure activity are different in adult and juvenile rats, we tested the ability of hippocampal slices prepared from kainate‐treated juvenile rats to exhibit long‐term potentiation. Long‐term potentiation was induced by theta‐burst stimulation and was not significantly different in slices prepared from control or kainate‐injected juvenile rats (16–18 postnatal days). Short‐term potentiation, however, was reduced in the kainate‐treated juveniles. Calpain inhibitor I has been shown to prevent long‐term potentiation formation in adult hippocampal slices, and we evaluated its effect on long‐term potentiation in hippocampal slices from juvenile rats. Calpain inhibitor I produced a significant reduction in the degree of long‐term potentiation induced by theta‐burst stimulation in hippocampal slices prepared from 14–20 postnatal day‐old animals. The results are consistent with the notion that, although similar mechanisms participate in the formation of long‐term potentiation in juvenile and adult animals, juvenile animals are much more resistant than adult animals to the consequences of seizure activity.