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Ontogeny of β‐adrenergic receptor‐mediated cyclic AMP generating system in primary cultured neurons
Author(s) -
Ma FuHai,
Ohkuma Seitaro,
Kishi Masataka,
Kuriyama Kinya
Publication year - 1991
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(91)90056-r
Subject(s) - forskolin , adenylate kinase , cyclase , medicine , carbachol , endocrinology , muscarinic acetylcholine receptor , biology , gtp' , agonist , receptor , g protein , chemistry , biochemistry , stimulation , enzyme
The developmental changes in the β‐adrenergic receptor/cyclic AMP generating system were examined using mouse cerebral cortical neurons in primary culture. During neuronal growth in vitro , the number of binding sites for [ 3 H]dihydroalprenolol (DHA) showed a tendency to increase ( B max ), while the affinity ( K d ) for [ 3 H]DHA did not show any noticeable changes. Basal and isoproterenol‐stimulated adenylate cyclase activities as well as the activation of adenylate cyclase by 5′‐guanylylimidodiphosphate (GppNHp), NaF and forskolin showed progressive and parallel increases during neuronal growth on a polylysine‐coated surface. The treatment of primary cultured neurons with islet‐activating protein (IAP), one of the pertussis toxins, attenuated the inhibitory effect of carbachol, a muscarinic agonist, on isoproteronol‐induced activation of adenylate cyclase activity. These results indicate that primary cultured neurons possess a cyclic AMP generating system coupled with β‐adrenergic and muscarinic receptors, which is regulated via stimulatory and inhibitory GTP‐binding proteins, respectively. The results described above also suggest that the β‐adrenergic receptor, stimulatory and inhibitory types of GTP‐binding proteins and adenylate cyclase may develop in a parallel fashion during neuronal growth on a polylysine‐coated surface.