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Influence of development and aging on nicotinic receptor subtypes in rodent brain
Author(s) -
Zhang Xiao,
Wahlström Göran,
Nordberg Agneta
Publication year - 1990
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(90)90065-a
Subject(s) - nicotine , nicotinic agonist , acetylcholine receptor , binding site , nicotinic acetylcholine receptor , chemistry , receptor , alpha 4 beta 2 nicotinic receptor , population , rodent , endocrinology , biology , medicine , biochemistry , neuroscience , ecology , environmental health
The influence of development and aging on nicotinic receptor subtypes in rodent brain was investigated. 3 H‐nicotine and 3 H‐acetylcholine ( 3 H‐ACh) were used as receptor ligands. Specific binding sites for 3 H‐nicotine and 3 H‐ACh were detected in mouse brain during the late prenatal period. A drop in the number of 3 H‐nicotine and 3 H‐ACh binding sites was measured shortly after birth. The 3 H‐nicotine and 3 H‐ACh binding sites showed different time courses during prenatal development. Competition experiments using unlabelled (−)nicotine and 3 H‐nicotine revealed one population of high affinity nicotinic binding sites in the cortex of 1‐day and 5‐day‐old mice whereas both a set of high and low affinity binding sites were found in adult mice. The proportion of cortical high and low affinity nicotinic binding sites did not change with aging although the absolute amount of high affinity nicotinic binding sites decreased. The 3 H‐nicotine binding showed different temperature dependence in rat brain of different ages. The results illustrate dynamic changes in nicotinic receptor properties during life span of rodents.