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Immunocytochemical study of S‐100 positive glial cells in the brainstem and spinal cord of the rat embryo
Author(s) -
Gomez Luciano Ariel,
Brusco Alicia,
Saavedra Jorge Pecci
Publication year - 1990
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(90)90023-u
Subject(s) - brainstem , spinal cord , immunocytochemistry , biology , neuroglia , embryo , embryonic stem cell , anatomy , central nervous system , medulla , neuroscience , microbiology and biotechnology , endocrinology , biochemistry , gene
A light and electronmicroscopic immunocytochemical study of the glial cells in the brainstem and spinal cord of the 18th day rat embryo was performed using an anti‐S‐100 protein antiserum. Only the radial glia and the free immature glial cells are S‐100 immunoreactive. Neurons are devoid of S‐100 immunoreactivity. The radial glia form two paramedial plates and a great number of lateral plates, uniformly spaced along the ventral portion of the brainstem from the mesencephalon to the medulla. The S‐100 protein was also detected in the perivascular membranes and glial limitans. Embryonic glia adopt a highly organized spatial pattern in the brainstem that could set the structural basis for an organized assembly of the developing nervous tissue. The use of the S‐100 protein as a glial marker in the embryonic rat brain proved to be of great value. Antibodies to S‐100 protein allow the demonstration of immature glial cells and a highly organized spatial pattern in the brainstem and spinal cord of the rat embryo.