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Nerve growth factor induces neurite outgrowth in a clone derived from an NGF‐insensitive human neuroblastoma cell line
Author(s) -
Reynolds C. Patrick,
PerezPolo J. Regino
Publication year - 1989
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(89)90063-4
Subject(s) - nerve growth factor , neurite , neuroblastoma , clone (java method) , cell culture , biology , microbiology and biotechnology , neuroscience , medicine , endocrinology , in vitro , receptor , genetics , gene
Nerve growth factor (NGF) plays a trophic and tropic role in the development of vertebrate sympathetic and sensory ganglia; however, the precise nature of the NGF effect(s) is not understood. Study of NGF‐responsive human neuroblastoma cell lines allows characterization of NGF‐induced neunte outgrowth in cells not dependent on NGF for survival. The human neuroblastoma line SK‐N‐SH did not significantly extend neuntes in response to NGF, but did show an increase in cell number. By contrast, a clone of the line, SH‐IN, extended neuntes in response to NGF or dibutyryl cyclic AMP, and showed inhibition of cell proliferation. Thus, cells capable of morphological differentiation in response to NGF can be cloned from neuroblastoma cell lines in which the majority of the cells fail to extend neurites even in the presence of NGF.