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In vitro experiments on neuronal and glial cell lineages among the ventricular cells of the mouse neural plate
Author(s) -
Buse Eberhard,
KindlerRöhrborn Andrea,
Rajewsky Manfred F.
Publication year - 1989
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(89)90049-x
Subject(s) - biology , progenitor cell , embryonic stem cell , microbiology and biotechnology , neural stem cell , neuroepithelial cell , cell type , in vitro , cell culture , cellular differentiation , immunohistochemistry , polyclonal antibodies , neuroglia , neuroscience , cell , antibody , stem cell , immunology , central nervous system , genetics , gene
The proliferative ventricular cells of the early neural plate of the mouse are generally assumed to be pluripotent and equivalent to one another in their developmental capability. Ventricular cells from the rostral parts of the neural plates of mice (Theiler stages 11 and 12, embryonic days 7 1/2 and 8) were studied in tissue culture with respect to their potential to give rise to neurons or glial cells, or both. Auto‐radiographic and immunohistochemical analyses showed that ventricular cells developing into neuronal phenotypes stopped proliferating immediately upon transfer to cell culture. Using polyclonal anti‐GFAP antibodies, a small proportion of immunoreactive cells could be detected after 4 days of culture. These cells retained their proliferative activity, displayed morphological characteristics of radial glial cells and may have either developed from specific glial progenitor cells or have been induced to proceed along the glial differentiation pathway at the beginning of culture. Therefore, two distinct types of progenitor cells, committed either to neuronal or glial lineages, appear to co‐exist among the cultured neural plate ventricular cells.