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Antioxidant enzymatic activities and resistance to oxidative stress in primary and subcultured rat astroglial cells
Author(s) -
Vanella A.,
Avola R.,
Condorelli D. F.,
Campisi A.,
Costa A.,
Stella A. M. Giuffrida,
PerezPolo J. R.
Publication year - 1989
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(89)90028-2
Subject(s) - subculture (biology) , oxidative stress , antioxidant , enzyme , chemistry , radical , microbiology and biotechnology , free radical theory of aging , biochemistry , biology
The survival of neural tissues depends in part on the balance between the formation of free radicals due to oxidative metabolism and the transformation of the free radicals to non‐toxic compounds. Serial subculture of rat glial cells as described here resulted in a decrease of the specific activities of several antioxidant enzymes and a glial specific marker for astrocytes. Thus, there was an increased susceptibility to oxidative stress in cultures by the third passage. These subcultured glial cell cultures may represent a useful model for the study of free radical induced neural damage that may be relevant to CNS trauma and aging.