Premium
Plasminogen activator secretion in relation to Schwann cell activities
Author(s) -
Krystosek Alphonse,
Verrall Shahla,
Seeds Nicholas W.
Publication year - 1988
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(88)90054-8
Subject(s) - plasminogen activator , urokinase , cell culture , secretion , biology , schwann cell , microbiology and biotechnology , motility , biochemistry , endocrinology , genetics
The molecular characterization of neural secretory plasminogen activators and their possible association with two well‐defined Schwann cell activities (mitosis and migration) were investigated. Schwann cells from peripheral sensory ganglion roots were established as primary cultures of high purity. Conditioned culture medium was fractionated by gel electrophoresis and assayed for plasminogendependent in situ caseinolytic activity. Both tissue‐type and urokinase‐type plasminogen activators were detected early in culture. The amount of the tissue‐type form decreased with increasing time in culture. Proliferative ability measured as [ 3 H]thymidine incorporation into the nucleus varied under several culture conditions and was lowest in defined serum‐free medium; however, these serum‐free cultures expressed the highest level of plasminogen activator secreting cells. Combined autoradiography/fibrin overlay assays allowed for a direct analysis at the single cell level; interestingly the findings did not support the idea of an obligatory expression of the enzyme in replicating cells. Cell motility was assayed in combined gold particle clearing/fibrin overlay assays and likewise no obligatory association of protease secretion and cell movement could be demonstrated. The results are discussed in relation to previous tudies in this field.