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Paradoxical sleep deprivation of the mother enhances DNA synthesis in fetal rat brain: Autoradiographic and biochemical evidence
Author(s) -
Zucconi Giglola Grassi,
Belia Silvia,
Menichini Enrico,
Castigli Emilia,
Giuditta Antonio
Publication year - 1986
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(86)90042-0
Subject(s) - sleep deprivation , sleep (system call) , maternal deprivation , fetus , neuroscience , endocrinology , biology , dna synthesis , psychology , dna , medicine , microbiology and biotechnology , genetics , pregnancy , circadian rhythm , computer science , operating system
Pregnant rats were deprived of paradoxical sleep for 3 days starting on the 18th gestational day. The condition of PS‐D was imposed by confinement on a small platform surrounded by water or by daily injections of clomipramine. Four hours before the killing rats received a s.c. injection of [ 3 H]‐thymidine. The amount of radioactive DNA determined by autoradiography in several regions of fetal brain was found to be markedly increased under both experimental conditions in comparison with the control fetal brain. Considerably more limited effects were observed in kidney. Comparable changes of lower magnitude were obtained by comparing the specific radioactivity of DNA samples purified by chlorophorm extraction and digestion with RNase and proteinase K. The results fully confirm our previous data obtained under similar experimental conditions but based on the analysis of an acid‐washed DNA fraction.