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Free amino acids of rat astrocytes in primary culture: Changes during cell maturation
Author(s) -
Holopainen I.,
Oja S. S.,
Marnela KirsiMarja,
Kontro Pirjo
Publication year - 1986
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(86)90031-6
Subject(s) - taurine , glutamine , amino acid , primary culture , intracellular , cell culture , biochemistry , biology , medicine , astrocyte , primary (astronomy) , endocrinology , chemistry , central nervous system , genetics , physics , astronomy
The concentrations of free amino acids were analysed in cultured primary astrocytes during cell maturation and in the starting material, i.e. the cerebral hemispheres of newborn rats. Taurine was the most abundant amino acid in all samples, the content of glutamine being comparable only in immature astrocytes (7 days in culture). The intracellular levels of most amino acids significantly decreased during the first 2 weeks in culture, remaining fairly stable during the third week.