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Acetylcholine sensitivity in myotubes of nerve‐muscle co‐culture cultured with anti‐muscle antibodies, α‐bungarotoxin and d ‐tubocurarine
Author(s) -
Kimura M.,
Shikada K.,
Nojima H.,
Kimura I.
Publication year - 1986
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(86)90017-1
Subject(s) - myogenesis , bungarotoxin , d tubocurarine , acetylcholine , acetylcholine receptor , antibody , myocyte , chemistry , medicine , endocrinology , biology , biochemistry , receptor , pharmacology , immunology
The effects of α‐bungarotoxin (α‐BuTX). d ‐tubocurarine ( d ‐TC) and antibodies against muscle extract on acetylcholine (ACh) sensitivity were investigated in developing mouse myotubes in a nerve‐muscle co‐culture. Antibodies clearly suppressed the ACh potential amplitude in adult mouse diaphragm muscle, but antibodies in muscle pre‐treated with d ‐TC (1 μg/ml) weakly suppressed it. The addition of d ‐TC to muscle extract dose‐dependently inhibited the formation of the immunoprecipitation lines. The exposure of developing myotubes to antibodies for 10 days in culture suppressed both resting potential and ACh potential, whereas co‐existence of α‐BuTX (1 μg/ml) or d ‐TC (0.1 mg/ml) with antibodies suppressed ACh potential but did not affect resting potential compared with antibodies alone. The ACh potentials in myotubes cultured with α‐BuTX and d ‐TC alone were also suppressed. The appearance (day 8 in culture) of this suppressive effect by α‐BuTX was faster than that (day 11 in culture) of d ‐TC. These different effects depending on the time in culture may account for the conformational change of developing ACh receptors to α‐BuTX and d ‐TC.