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Development of neuronal markers in aggregating fetal rat telencephalon cells cultured in the presence of triiodothyronine
Author(s) -
Jørgensen Ole Steen,
Honegger Paul
Publication year - 1986
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(86)90004-3
Subject(s) - cerebrum , triiodothyronine , nerve growth factor , choline acetyltransferase , medicine , endocrinology , cholinergic neuron , enolase , biology , cholinergic , stimulation , central nervous system , hormone , immunohistochemistry , receptor
The concentrations of the general neuronal markers D2‐protein (N‐CAM), D3‐protein and neuron specific enolase (NSE) in reaggregating cultures of fetal rat telencephalon cells were affected by the presence of 30 nM triiodothyronine in the defined culture medium. The extent of normal developmental changes were enhanced by triiodothyronine, as demonstrated by crossed immunoelectrophoresis. From 13 to 19 days in culture, the concentration of D2‐protein decreased, and the concentrations of both D3‐protein and NSE increased. Nerve growth factor (NGF) was without effect on the development of these general neuronal markers. However, as shown previously both triiodothyronine and NGF increased the activity of choline acetyltransferase, a marker for cholinergic neurons. The results suggest an enhanced overall differentiation of several types of telencephalon neurons in the presence of triiodothyronine, and a specific stimulation of cholinergic telencephalon neurons by NGF.