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Regional effects of the convulsant methionine sulfoximine on the benzodiazepine receptor complex of rat brain
Author(s) -
Sellinger O.Z.,
Gregor P.,
Sellinger A.
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90258-8
Subject(s) - neurochemistry , mental health , convulsant , library science , psychology , neuroscience , medicine , neurology , psychiatry , receptor , computer science
We used an antibody to L-glutamic acid decarboxylase (GAD), a synthesizing enzyme for GABA, to immunocytochemically localize GABAergic neurons in the developing C57BL/6J mouse retina. At early developmental stages (embryonic day 17 to postnatal day 3) strong GAD-immunoreactivity is detectable in cell bodies located within the neuroblastic layer. These relatively large cells with sturdy, radially oriented processes could be identif ied as developing horizontal cells. In addition the inner plexiform layer (IPL) and cell bodies adj6~cent to it have weak GAD-immunoreactivity. By postnatal day 6 GAD-posit ive horizontal cell processes begin to form a horizontal network in the newly formed outer plexiform layer (OPL), and immunolabeling of amacrine cell bodies and of the IPL became~; much stronger. During the second postnatal week GAD-posit ive material in the IPL becames stratif ied and the GAD-immunoreactivity of amacrine cells reaches a maximum. Amacrine cells remain im/nunoreactive into adulthood as does the IPL. However, after postnatal day 12 GAD-immunoreactivity of the horizontal cells begins to decline; in 4-week-old mice the horizontal cells are no longer detectably labeled by GAD antiserum. The function of this transient GAD in horizontal cells during early development remains to be elucidated.