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Oligodendrocytes in primary dissociated culture accumulate 3 H‐gaba but not other amino acid transmitters
Author(s) -
Reynolds Richard,
Herschkowitz Norbert
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90255-2
Subject(s) - citation , primary culture , library science , dept , medicine , psychology , cell culture , chemistry , computer science , biology , stereochemistry , genetics
1 A growth cone enriched fraction was prepargd from (Pfi) ra~ forehrain . This fraction has a sodlum-dependent, high affinity upta~e system for [~H] GABA with a K of 4.5~M m and a Vmax of 59.4 pmol/min/mg. Electron microscopic autoradingraphy unequivocally demonstrated the abil i ty of isolated growt~ cones to take up [3H] GABA. Superfuslon studies of isolated grgwth cones pre-labelled with [3HI GARA demonstrated a K + (25mM) induced release of [SH] GABA which at its peak w~s twice the pre-stimulus release level. About 50% of the K * induced rele~ase of [3HI GABA was Ca ++ dependent.