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GABA Transport systems in cultured cerebellar astrocytes
Author(s) -
Johnstone Stephen R.,
Wilkin Graham P.,
Levi Giulio,
Ciotti M. Teresa
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90252-7
Subject(s) - neuroscience , cerebellum , astrocyte , chemistry , biology , central nervous system
Dissociated cell cultures of fetal rat hippocampus were maintained for 8 45 days in vitro (DIV) . The time course of neuronal differentiation could be followed by immunocytochem:.cal detection of neuron-specific enolase (NSE) . Simultanous demonstration of glutamate decarboxylase (GAD) and somatostat~n (SOM)-like immunoreactivity was performed by a combination of the peroxidase-anti peroxidase method and immunofluorescence. The highest number of GAD containing cells (about 0.7 %) could be observed at 30 DIV. A fraction of GAD neurons additionally exhibited SOM-like immunofluorescence. The highest number of SOM neurons was also found at the age of 30 DIV. GAD-negative SOM cells could not be observed. It is likely that GABAergic neurons develop from precursors of basket cells and other interneurons and that they are divided into several subclasses each of which contains a different peptide. The time course of the appearance of NSE does not coincide with the development of neurotransmitter/neuromodulator specificity. Supported by the DFG (SFB 87, B 11).