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Development of 3 H‐GABA uptake and evoked release in cerebellar interneuron‐enriched primary cultures
Author(s) -
Aloisi Francesca,
Levi Giulio
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90245-x
Subject(s) - interneuron , neuroscience , biology , inhibitory postsynaptic potential
Primary cultures from dissociated 8-day postnatal rat eerebellar cells contain a subpopulation (2%) of neurons which are able to ~ceumulate 3H-GABA and to release it in a Ca2+-dependent manner upon depolarization. )ptake and release developed gradually. At 2 DIV scarse 3H-GABA uptake and no Ca2+-dep9ndent, evoked release could be detected. At 5 DIV GABAergic neurons exhibited a strong accumulation of 3H-GABA over the pericarya and the developing axonal processes, and a large Ca2+-dependent release could be evoked by high K +. At later stages the progressive increase in branching and labeling of the axonal processes was paralleled by a furthe ~ increase in the amount of 3H-GABA released. The 3H-GABA accumulating stellate astrocyte:3 present in the cultures were not responsible for the 3H-GABA evoked release. In fact the magnitude of the evoked release depended on the number of GABAergie neurons in the cJlture. This number could be 90% reduced by subjecting the cultures to a medium change at day 7. The decrease of GABAergic neurons could be attributed to a low molecular wei~it (