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Electrophysiological characteristics of the cultured purkinje cells: A quantitative analysis
Author(s) -
Calvet M.C.,
Calvet J.
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90212-6
Subject(s) - computer science , library science
The spontaneous and evoked electrical ac t iv i ty was extracel lu lar ly recorded from Purkinje (P) cells grown in di f ferent ly organized explants aged of at least 30 DIV. Electrophysiological data were processed with a LS1 11 computer programmed to display standard interspike interval histograms bi-histograms and cross-correlograms. When the cerebellar explant developed in co-culture with brain stem tissues, cross-correlation analysis indicated that 80 % of the P. cell pairs had a tendency to f i re synchronously. No correlation could be evidenced between two P. cells grown without their extracellular afferents. A high degree of synchronization was also found between P. cells and brain stem neurons. Depending on the brain stem stimulating site di f ferent types of P. cell responses were individualized. The onset of climbing f iber function in P. cell electrogenesis in v i t ro is specially discussed.