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Serum and hormones effects on enzymes of energy metabolism in cultured chick neurons and glial cells
Author(s) -
Tholey G.,
Ledig M.,
Bloch S.,
Mandel P.
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90206-0
Subject(s) - hormone , energy metabolism , citation , enzyme , physics , endocrinology , biology , computer science , library science , nuclear magnetic resonance
Homogenous primary cultures of astrocytes and neurons obtained from chick embryos were performed in a serum free medium. In these conditions maturation phenomena affecting part icular ly the energy metabolism of the nerve cells were observed. A decrease (-82%)of M subunit containing lactate dehydrogenase and an increase (+ 105 %) of neuron specific form of enolase (NSE) act iv i t ies were observed in neurons grown in a serum free medium as compared to serum supplemented controls. Glial cells grown in absence of serum contained higher values of non neuronal enolase (NNE) and glutamine synthetase (GS) than controls. Addition of hydrocortisone to the culture medium increased s ign i f icant ly the levels of NNE and GS in neurons and g l ia l cells respectively. The phenomenon is more pronounced in the absence of serum. Addition of steroid hormones to the serum free medium decreased specif ical ly the levels of NNE, NSE and GS in neurons and g l ia l cells respectively. I t is suggested that some inhibi tory effects observed with serum may be attributed to this type of hormones.