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Regulation Of Translation In Cox Astrocytoma Cells Grown In The Presence of Ethanol
Author(s) -
Tewari Sujata,
Daly Cindy,
Woodson Mark
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90205-9
Subject(s) - citation , translation (biology) , library science , computer science , biology , genetics , gene , messenger rna
The use of tow concentrations of fetal calf serum in cultures of di f ferent ages of C 1300 mouse neuroblastoma, clone neuro-2a, has produced morphological developmental alterations which included presynaptic elements in the form of varicosities or terminal swellings. The lat ter ware f i l led with round to oval, clear vesicles, the size ranging from 40-200 nm. The use of a defined medium did not in any way affect the morphological development of these cells. Addition of ~-aminobutyr ic acid (GABA) or sod_~m brorr~ide (NaBr) to the culture medium at concentrations ranging from 10 t o 10-~M over short periods of t ime (2 days), produced increased plast ici ty of the neurites, accompanied by the presence of immature synapticl ike junctions. Application of a mixture of bovine brain gangliosides consisting of 27%GMI, 40%GDla, 16% GDIb and 19% GT to GABA or NaBrcondit ionedcells, induced further morphological di f ferent iat ion which was accompanied by mature synapse-like contacts. No such contacts could be detected when the GMI fract ion was removed from the ganglioside mixture. Time-lapse cinematography revealed that gangliosides contributed elaborate contacts of long duration to the stable system. With the support of German Science Foundation Grant Sp237/2-1 .