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Sympathetic survival factors increased by denervation
Author(s) -
Hendry I.A.,
Kanakis S.J.,
Hill C.E.,
Watters D.J.
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90191-1
Subject(s) - medical school , library science , medicine , political science , medical education , computer science
Sympathetic denervation of rat heart by administration of 6-hydroxydopamine (6OHDA) results in an increase in the activity of factors required for the survival of 12 day old chick embryo lumbar sympathetic ganglien neurones in dissociated cell culture. A large proportion of this increased activity can be blocked with affinity purified antibodies to mouse salivary gland nerve growth factor CNGF). In order to further investigate this increase we have fractionsted extracts of normal and denervated heart using both gel filtration on sephadex G-100 and immuno-affinity chromatography with antibodies to mouse NGF linked to sepharose. In control heart there are two components with apparent molecular weight on sephadex G-100 of 40,000 and 12,000 that have sympathetic survival activity but neither of these is NGF. After denervation, NGF can be detected at a level of 1.6 ng/gm in the heart and comprises the major component of the increased survival activity. In addition the two other components are increased in activity by 200% and 4504 respectively. ThSs suggests that all three components are regulated by innervation either by a direct action on their synthesis or by removal due to retrograde axonal transport. Alternatively NGF is only important during initial development and regeneration while the other two components are more important for the on-going maintenance of sympathetic neurones.