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Developmental changes of some enzymes related to energy transduction in free and synaptic mitochondria of rat brain
Author(s) -
Curti D.,
Dagani F.,
Marzatico F.,
Zanada F.,
Lombarde B.
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90185-6
Subject(s) - technical university , library science , computer science
The changes of some enzymes related to energy transductJon in rat brain subcellular fractions at 5, i0, 15, 20 and 30 days of postnatal development was investigated. The following enzyme activities in free and synaptic mitochondrJa obtained from cerebral hemispheres and cerebellum were evaluated: citrate synthase (EC 4.1.3.7); succ~nate dehydrogenase (EC 1.3.99.1); rotenone insensitive NADH-cytochrome c reductase (EC 1.6.99.3) cytochrome oxJdase (EC 1.9.3.1); glutamate dehydrogenase (EC 1.4.1.3). The activities of lactate dehydrogenase (EC 1.1.1.27), acetylcholJne esterase (EC 3.1.1.7) and cytochrome oxidase were measured on synaptosomes before lysis. The specific activities of c~trate synthase, glutamate dehydrogenase, cytochrome oxJdase Jn free mJtochondrJa increased during development. A different pattern of these enzymatic activities in fraction enriched Jn synaptic mJtochondrJa was observed.