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Molecular species composition and acyl group biosynthesis of myelin glycerophospholipids in the rat sciatic nerve during regeneration and development
Author(s) -
Alberghlna M.,
Viola M.,
Giuffrida A.M.
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90180-7
Subject(s) - glycerophospholipids , library science , regeneration (biology) , biology , biochemistry , computer science , microbiology and biotechnology , phospholipid , membrane
Polyclonal antibodies against neurofilament (NFP68), GFAP, Neurin and monoclonal antibodies against glia-processes (XlXDI,XXIXBI) were used for the study of neuron and glia differentiation within the developing and adult fish spinal cord. NFP68 reactivity appears in primary sensory neurons at 60 h post fertilization (pf), and in motoneurons at 72 h pf. Neurin is present in neuronal and glial cells at 60 h pf, and GFAP reactivity appears in radial glial cells at 60 h pf. XIXD| and XXIXBI reactivity is present in all embryonic spinal cells at 84 h pf, in adults however, it appears only in radial and longitudinal glial processes. Conclusions: I) Neurin is an early and potent marker for embryonic glial cells and for spinal neurons; 2) with regard to the markers mentioned above, the primary sensory system and the radial glial cells differentiate prior to the spinal motor system; 3) neurons and glial cells have a common marker in embryonic stages, which in adults is only expressed in glial processes.