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A new neurone specific developmentally modified antigen of rat cerebellum
Author(s) -
Gombos G.,
Ghandour M.S.,
Foucaud B.,
Langley O.K.
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90176-5
Subject(s) - citation , library science , humanities , physics , computer science , art
In the P.N.S., GFAP fi laments have been detected Immmunohlstochemlcal ly In the enteric 91101 cel Is and somme Schwann ce l l s . ItBre we study the molecular ident i ly of th is P.N.S. GFAP Immunoresctlvlty, and compare I t lo GFAP in the C.N.S., using Immuno¢hemlcal and ImunGhlslDchemical methods. Immunoblafftlng, with antiserum lo brain GFAP, shows that the peripheral GFAP I lmunore~ct lv l ly res i des in a polypeptlde with • molecular weight of 49 kdw identical lo that of r a t brain GFAP. Furthermore, th is GFAP reac t i v i t y can he detected Ilmunahlsl~chemlcel ly in Schwann and satel l i f e ce l l s in several peripheral nerves and ganglia, in addit ion to enteric g i l a . The d l s l r l bu t i on of GFAP among Schwann eel Is suggests that , In the nerves surveyed, they may be expressed by most or al I non-myelin forming Schwann cel Is, but not by myelln-for~lng Schwann eel Is. We also show, using a monoclonal antibody to GFAP (antI-GFAP-3, made by Albrachtsen, GJrster4)erg end Beck) In both Immunohlslochemlcal end Imunoblott lng sl~dlws, that the GFAP found in most peripheral g i la Is not identical to that of eslTocytes. Act exception lo th is f inding Is seen in the myenteric plexuses where Immunohlstochemlcal ly detectable GFAP Is found In aclep but not al I , of the enteric g i l a , using the monoclonal antibody. 111us, the rmu i t s suggest that GFA polyl)eptldes may be a heterogenous group, that share some common deter~inants and a cmmon molecular weight, and show a widespread end complex dls lTIbut lon In the g i la of both the C.N.S. and P.N.S.