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S‐100 immunoreactivity in foetal rat skin
Author(s) -
Rende M.,
Michetti F.,
Stolfi V.M.,
Battaglia F.,
Ladogana A.,
Cocchia D.
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90168-6
Subject(s) - biology , human physiology , humanities , philosophy , endocrinology
Previous studies utilizing pharmacologic blockade of the embryonic neuromuscular transmission have suggested that none of the known components of the neuromuscular transmission seem to be essential for the formation of the neuromuscular synapses. In the present study the role of motor nerve fibers in formation of the postsynaptic specializations was examined by studying muscle cells developing in vivo in the absence of motor nerve. The superior oblique muscle of duck embryos was made aneural by permanent destruction of the trochlear notor neurons on day 7, three days before the nerve fibers normally reach the muscle Absence of the innervation was verified by light and electron microscopy of the muscle. Synaptic folds, postsynaptic density and basal lamina were often seen on muscle cells developing in the absence of motor neurons. Further, basal lamina differentiated into synaptic and extrasynaptic portions as detected with a monoclonal antibody. The high molecular weight form of acetylcholinesterase was present in the aneural muscle and electron microscopy showed that the reaction product was associated w2th the basal lamina. Further, hyperinnervation did not affect the total number of synapses per muscle. The results suggests that the mechanism for the development of postsynaptic specializations may be preprogrammed in the muscle cell. (Supported by grants from NIH).