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Neuroimmunology of human fetal brain in tissue culture
Author(s) -
Silani V.,
Buscaglia M.,
Scarlato G.
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90165-0
Subject(s) - medical school , obstetrics and gynaecology , medicine , library science , family medicine , medical education , pregnancy , biology , computer science , genetics
Sensory roots were studied two months after coagulation. On the deafferented side the cross section surface area was reduced of 31% as compared to the normal side. Myelinated fibres were coun~d on light micrographic montages of semithin cross sections. The total number of myelinated fibres was decreased of 21% on the deafferented side. The frequency distributions of myelinated axon diameters indicated that the f~b~es lost were essentially large ones, and were situated in the medial part of the sensory root. The relative proportion of myelinated and unmyelinated fibres counted on electron micrographs was unchanged. This loss of axons found in the sensory root was related to neuronal death previously described by us in the trige~inal ganglion. Signs of axonal regeneration were not found in the deafferented root two months after vibrissae coagulation. A morphometric study of subnucleus interpolaris of the spinal trigeminal complex was performed. This vibrissal relay was shrunken on the coagulated side and no sign of trans-synaptic degeneration could be seen at this level, one month after deafferentation. A qualitative and quantitative EM study of synapses is in progress.