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Structural and functional changes of n‐cam protein during development
Author(s) -
Gennarini G.,
Hirn M.,
Rougon P.,
Sadoul R.,
Goridis C.
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90159-5
Subject(s) - humanities , philosophy
Sy-1 is a soluble brain-specific protein recently detected and partially characterized in rat brain (1). By means of a monospecific anti-Sy-1 serum the immunocytochemical localization of the protein in rat cerebella of 8, 12, 15, 21 and 90 postnatal (pn) days was studied. At the end of the first pn week the labelling is exclusively present in the cytoplasm of Purkinje cells (PC). During the second pn week the staining becomes even more intense in both the soma and the dendritic tree of the PC; the peroxidase product appears also in the cytoplasm of Golgi neurones, stellate and basket cells. No marked differences were noted between the pattern of immunolabelling at 15 and 21 or 90 pn days. Granule cells appear free of immunostain at any tested age; control sections (prepared utilizing an irrilevant antiserum) were never labelled. Doublelabelling experiments, Sy-1 is not present in