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A method for the study of neural cell lineages, based on colony cultures and transplantation of cultured cells into CNS
Author(s) -
Fedoroff S.
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90141-8
Subject(s) - citation , transplantation , library science , biology , psychology , computer science , medicine
Proliferative neural cells can be isolated at various stages of development by using developmentally staged embryos or postnatal animals and colony cultures. Proliferative cells are distributed according to the Poisson distribution in disaggregated neural tissue cell suspensions and when planted in cultures they adhere to the substratum and proliferate forming colonies composed of closely related cells. The colonies have characteristic gross morphology related to their cells and the number of colonies formed is in direct proportion to the size of the inoculum. Specific cell types can be isolated from the colony cultures by picking up colonies or by using metrizamide density gradients. The enriched specific cell populations can be studied under various conditions in tissue culture or in transplants into the CNS. In tissue culture as well as in transplants the cells progress through their predetermined lineage of differentiation. Using this procedure it has been possible to isolate early neuronal precursor cells before they expressed specific ~~unological markers or morphological characteristics and to follow the sequence of their differentiation. Supported by MRC Canada Grant MT-4325.