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Prenatal hypoxia on brain development: dna, rna, protein and cerebroside parameters
Author(s) -
Yu Mang C.
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90130-3
Subject(s) - citation , cerebroside , library science , psychology , law , political science , computer science , neuroscience
In order to analyze in the optic tectum the development of the blood-brain barrier (BBB) to horseradish peroxidase (HRP) two series of chick embryos of 10 and 16 incubation days were intracardially injected 0,3 mg HPR (Sigma type II) dissolved in 0,l ml saline per gram body weight. In the first group fertilized chicken eggs were incubated under routine conditions, in the second, the egg shell was partly covered with a layer of paraffin so as to obtain an impediment to the normal exchanges between embryo developing blood vessels and the outside environment.The specimens were sacrificed 3 and 20 minutes after injection. In the tectum the BBB to HRP is established on the 16th incubation day when the endothelial basement lamina, the astrocyte perivascular sheath and the tight junctions between neighbouring endothelial cells are formed. The O2 deprivation, which progressively leads to neuronal death, apparently does not affect the development of the BBB to HRP since, under hypoxic as well as normal conditions, at the 10th incubation day the reaction product of the marker is recognizable throughout the layers of the grey and white matter, while, at the 16th incubation day, it is mainly confined within the blood vessel lumina.