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Influence of n‐isopropylamino‐2‐pyrimidine and its derivatives on neuronal growth and adenosine release
Author(s) -
Ciccarelli R.,
Di Iorio P.,
Laddomada A.,
Calabrese S.,
Caciagli F.
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90126-1
Subject(s) - medicine
In contrast to mouse and chicken astrocytes the ganglioside pattern of cultured rat astrocytes is usually less differentiated. We have obtained apparently a line of rat astrocytes probably through a slight spontaneous transformation. The doubling-time of this cultures is 24 hours while that of primary cultures of rat astrocytes is 36 hours in similar conditions. After 40 generations the specific glial fibrillar acidic protein is still present in our astrocyte cultures as demonstrated by immunocytochemistry. A ganglioside pattern of differentiated cells with the presence of diand tetra-sialogangliosides was obtained. (Isolation and analysis of gangliosides were performed according to R.W. Ledeen & R.K. Yu, Methods in enzymology, 1982, 83, PP 139).