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A new cell surface glycoprotein involved in neurite outgrowth from the CNS
Author(s) -
HenkeFahle Sigrid,
Babiel Reiner
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90091-7
Subject(s) - citation , computer science , combinatorics , information retrieval , library science , bioinformatics , world wide web , computational biology , biology , mathematics
Monoclonal antibodies (MAbs) against molecules of cerebellar structures were obtained by injecting, as immunogens, into mice, cerebellar “membrane” fractions from 12 day old rats; the mice immunocytes were fused with cells from a mouse myeloma line and the hybridomae were cloned. Immunocytology with the MAbs produced by each hybridoma cell line and differential binding tests of these !4Abs to 12 day old and to adult membrane were used for screening and for selecting developmentally regulated antigens. Several of the obtained MAbs showed specificity for a single cell type (either neurones or astrocytes) or subtype (i.e. different neuronal types) or structures (i.e. the pinceau of basket cells, the parallel fibres, the glomeruli). Few MAbs reacted with developmentally regulated antigens. According to GJestern blots of SDS-PAGE electrophoresis of proteins derived from cerebellar membranes, from cytosol and from cytosqueleton fractions none of the epitopes revealed by our MAbs are localized in known CNS antigens.