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Regional [ 3 H]acetylcholine and [ 3 H]nicotine binding in developing mouse brain
Author(s) -
Larsson Christer,
Nordberg Agneta,
Falkeborn Ylva,
Lundberg PerÅke
Publication year - 1985
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(85)90057-7
Subject(s) - nicotine , hippocampus , nicotinic agonist , binding site , cerebellum , acetylcholine , cholinergic , endocrinology , medicine , cortex (anatomy) , acetylcholine receptor , chemistry , cerebral cortex , midbrain , biology , central nervous system , biochemistry , neuroscience , receptor
The postnatal development of nicotine‐like binding sites in the cortex, hippocampus, midbrain and cerebellum of 3‐, 7‐, 12‐, 17‐ and 30‐day‐old mice was studied. Two different nicotinic cholinergic ligands, namely [ 3 H]acetylcholine ([ 3 H]ACh) and [ 3 H]nicotine ([ 3 H]NIC) were used to detect the nicotine‐like binding sites in in vitro binding assays. The postnatal development of the binding sites of [ 3 H]NIC increased gradually with age in all brain regions studied. The [ 3 H]ACh binding, on the other hand, showed a marked peak on day 12 in the cerebellum and midbrain but did not change notably with age in the hippocampus and cortex, except for a slight temporary increase in the cortex on day 7. The time‐course for the appearance of nicotinic binding sites as observed with [ 3 H]ACh was found to be rather similar to that earlier described for [ 3 H]alpha‐bungarotoxin binding sites, whereas that for [ 3 H]NIC differed from that described for other nicotinic ligands.