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Viability of locus coeruleus cultures in the fourth ventricle of the adult rat
Author(s) -
Multon M.F.,
McRaeDegueurce A.
Publication year - 1984
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(84)90018-2
Subject(s) - locus coeruleus , tyrosine hydroxylase , immunocytochemistry , fourth ventricle , ventricle , catecholamine , biology , cerebral ventricle , transplantation , medicine , endocrinology , cisterna magna , dopamine , anatomy , neuroscience , central nervous system , cerebrospinal fluid
Both tyrosine hydroxylase and dopamine‐beta‐hydroxylase immunocytochemistry indicated the survival of catecholamine neurons and processes in 7‐day‐old organotype cultures of the locus coeruleus. Cultures of this age were transplanted in the 4th ventricle of adult rats via the cisterna magna. Two months after transplantation microscopic observations revealed that the transplant developed with a defined neural organization. Following tyrosine hydroxylase immunocytochemistry, immunoreactive cells were identified within the transplants. These results indicate that organotype cultures of the locus coeruleus survive within the 4th ventricle of adult rats.