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Evidence for the migration of principal and giant neurons into the developing dorsal cochlear nucleus of the north american opossum
Author(s) -
Willard F.H.,
Thompson D.M.,
Martin G.F.
Publication year - 1983
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(83)90372-6
Subject(s) - dorsum , state (computer science) , presentation (obstetrics) , citation , anatomy , library science , biology , medicine , computer science , radiology , algorithm
Serum-free co-cultures, consisting of h~nbar spinal ganglia and either dermal or epidermal explants from 7-day chick embryos, have been used to study the morphogenetic mechanisms involved in the establisNnent of cutaneous innervation. Preliminary results obtained from morphometric time-lapse analysis of the cultures demonstrate that nerve fibers behave differently when interacting with dermis or epidermis : I. When growing neurites meet migrating mesenchymal cells at the periphery of dermal explants, no change occurs in the general orientation of growth and transient contacts are formed between growth cones and dermal cells. 2. On the contrary, in the presence of epidermis, most nerve fibers (about 60%) change their orientation when arriving in the vicinity of epidermal cells and, at a mean distance of 40 p~n deviate around the explant. The growth cones of the rest of the fibers (about 40%) contact the epidermal cells but less than 10% end up inside the explant. It is the first time that such a "long range avoidance" is reported for nerve fibers.