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The effects of ploidy interactions on cell shape and size in the Xenopus optic tectum
Author(s) -
Szaro B.G.,
Tompkins R.T.
Publication year - 1983
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(83)90365-9
Subject(s) - optic tectum , citation , presentation (obstetrics) , xenopus , library science , biology , computer science , neuroscience , medicine , genetics , gene , central nervous system , radiology
Solutions of ammoniacal silver carbonate have been used for the selective staining of cell nuclei. We have now shown that embryonic neural cells engaged in DNA synthesis are differentially stained by this procedure. Three classes of nuclei can be recognized in the neural tube of chick embryos (stages 18-22). Neuronal nuclei show dark clumps of chromatin and a very pale matrix. They are identified by their position close to the marginal layer. The neuroepithelium presents dark and pale nuclei. Both types contain dark clumps of chromatin but only the former show a deep brown staining of their matrix. Both the incorporation of[JH] thymidine and the effect of cytosine arabinoside indicate that it is the dark neuroepithelial nuclei that are engaged in DNA synthesis. There is no such correlation between silver staining and DNA synthesis in the surrounding embryonic tissues.