Premium
Nascent neurotransmitter systems and the modulation of ontogenetic adaptations: serotonin and suckling
Author(s) -
Spear L.P.,
Ristine L.A.,
Scalzo F.M.
Publication year - 1983
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(83)90363-5
Subject(s) - library science , psychology , cognitive science , computer science
The adenylate cyclase system, which is the membranous relay between substances of the ex t race l l u la r mi l ieu and cAMP, the i n t r a c e l l u l a r messenger, play a s i gn i f i can t role in cel l migration and d i f f e r e n t i a t i o n during ear ly development. In recent studies we investigated the developmental p ro f i l e of the enzyme in the chick brain. We found that adenylate cyclase was present in the chick embryo gastrula and was markedly high from the 3rd through the 8th day of incubat ion. This embryonic period coincides with active neurogenesis and gl iogenesis. To explore fur ther the role of adenylate cyclase in neural growth and d i f f e r e n t i a t i o n , we have used neuronal-enriched cultures derived from 6-day-old chick embryo brain in DMEM (Dulbecco's Modified Eagle Medium) plus 10% FCS ( feta l ca l f serum), plated on polylysine-coated petr i dishes; and g l ia l -enr iched cultures from 15-day-old chick embryo cerebral hemispheres in DMEM plus 20% FCS, p la t ed on p las t ic pet r ic dishes. Both basal and NaF-stimulated adenylate cyclase a c t i v i t ies were present in both neuronaland g l ia l -enr iched cul tures as ear ly as 3 days in cu l ture. Prel iminary data show that cel l density has a dramatic e f fect on adenylate cyclase a c t i v i t y leve ls . Neural t issue cul ture is proving to be a valuable tool to study the role of adenylate cyclase in embryonic development. (Supported by research grants AA 03527 from NIAAA and DA 02131 from NIDA.)