Premium
Proliferation of rat CNS astrocytes in serum‐free defined medium and effect of different substrates on cell growth
Author(s) -
MichlerStuke A.
Publication year - 1983
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(83)90353-2
Subject(s) - citation , presentation (obstetrics) , cell growth , microbiology and biotechnology , computer science , biology , neuroscience , library science , medicine , biochemistry , surgery
Acom[s cahirinus (Spiny mouse) is a precocial murid, only recently used as experimental animal for anatomical and neurobehavioral studies. Its brain is characterized by a largely developed hippocampus with respect to neocortex if compared to the brain of other rodent species. This part icular feature appears of considerable interest for research on cholinergic, anticholinergic and anticholinesterase agents. In the present experiments choline acetyltransferase (CAT) and acetylcholinesterase (ACHE) were measured in whole brain from the birth up to 28 days at I week intervals using, respectively,3H-coenzyme A and acetylthiocholine as substrates. The rise of CAT was almost l inear from 90 up to 530 pmoles of ACh formed/mg of protein. Soluble AChE was almost constant up to 4 weeks--of about 1100-13OO mU(nmoles of AcThCh hydrolyzed/ min)/g of t issue and 26-30 mU/mg of protein. Total AChE rose from 1833 at birth to 2823 at 2 weeks and up to 3600 mU/g of tissue at 4 weeks with increase of specific activity from 28 to 40 mU/mg of protein. The density and aff inity of muscarinic receptor sites was also evaluated by selective 3H-quinuclidinyl benzilate binding. These aspects of development are discussed in direct comparison with those of the rat. Supported part ial ly by the Commission of European Comunities Contract 548 ENV-I (S)