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Lack of effects of neonatal hypothyroidism on lipid‐protein interaction in neuronal membranes
Author(s) -
Meisami E.
Publication year - 1983
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(83)90350-7
Subject(s) - citation , presentation (obstetrics) , library science , psychology , cognitive science , medicine , computer science , surgery
We have measured the spec i f ic binding of (3H)5-HT l(3H)Serotoninl to neuronal membranes from the spinal cord at d i f f e ren t postnatal ages and in the adul t . K D and Bmax were calculated from the Scatchard analysis of saturat ion curves. In the adul t K D and Bma x were: 17.5 ± 7.5 nM and 1.260 + 0.060 pmole/spinal cord. At zero days (b i r t h ) binding was already saturable with a K D of 16.3 ± 4 . 9 nM and a Bmax of 0.117 ± 0.029 pmole/spinal cord. Bmax increased with age being about eight times as much on day 21. A f f i n i t y did not change with age. Tryptophan-5-hydroxylase a presynaptic marker develops very s im i l a r l y suggesting the formation of spec i f ic synapses.