Premium
Duplication of positional information in regeneration of eye‐bud fragments results from a specific healing pattern
Author(s) -
Ide C.F.,
Reynolds P.,
Tompkins R.
Publication year - 1983
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(83)90336-2
Subject(s) - citation , computer science , gene duplication , presentation (obstetrics) , regeneration (biology) , information retrieval , world wide web , biology , genetics , medicine , gene , radiology
Fragment cultures of embryonic mouse neural tube were used to study sequential changes in neuronal cell differentiation. We e~amined expression of intermediate filaments (vimentin type, VIF; neurofilaments, NF) and related their patterns to the appearance of other phenotypic markers of neuronal development such as presence of tetanus toxin receptors (TI~, migration of cells, formation of Nissl substance, and the degree of neurite formation. Newly formed neurons, bipolar with scant Nissl substance, were close to the fragment (least migration). Tney were qTR-/NF-/VIF +, with VIF in a perinuclear arrangement e~tending into some processes. Neurons in the middle of the outgrowth zone (average migration) were TTR+/NF+-/VIF~-. Tne morphologically most differentiated neurons, large multipolar cells with abundant Nissl substance, appeared furthest from the fragment (extensive migration) and were TPR+/NF ~rfF-. Between days 7 and i0 of culturing neurons at all stages of differentiation were observable within the outgrowth zone. Neuronal migration appeared limited to the first 10-12 days of culture though neurons continued to differentiate throughout the culture period of 21 days. Tnis system thus provides a model by which mechanisms regulating neuronal proliferation and differentiation in vitro may be studied. Supported by MNC of Canada Grant MT 4235.