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Effect of corticosterone on cell cultures of chick embryo retina
Author(s) -
Gremo F.,
Porru S.,
Vernadakis A.
Publication year - 1983
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(83)90328-3
Subject(s) - medicine , library science , computer science
We have shown that in ovo treatment with corticosterone results in the appearance of a subpopulation of cholinergic muscarinic receptors which d i f fer signif icant ly from the controls. These data suggest that the hormone could affect cell surface as well. Retinal cultures were prepared from 8-day-old chick embryos, and at day 5 the medium (DMEM Dulbecco's Modified Eagle Medium) was replaced with DMEM + concentrations of corticosterone from lO -~ to lO -7 M. Controls received either DMEM + I0% fetal cal f serum (FCS) or DMEM only (serum-free). After 24 hr al l cultures received fresh DMEM + I0% FCS. Hormone-treated cultures showed a marked decrease in process development and a change in shape of neuronal aggregates. "Flat cells" showed increased prol i feration and morphological change. However, cells in serum-free cultures showed an increase in process sprouting during the period of serum deprivation. Within an hour after addition of serum, the processes were retracted. Thus, hormone-treated cultures differed signif icant ly from both control and serum-free cultures. These preliminary data support the hypothesis that the hormone directly affects cell surface, as well as causing intracel lu lar effects. (Supported by NATO grant no. 148.80. Dr. Gremo was a Visit ing Scientist and Mr. Porru a Visit ing Medical Student from the University of Cagliari School of Medicine, Cagliari, I ta l y . )