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Phase I and phase II reactions in brain and their involvement in neurotoxicity of selected chemicals
Author(s) -
Seth P.
Publication year - 1983
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(83)90311-8
Subject(s) - citation , neurotoxicity , library science , phase (matter) , computer science , toxicology , medicine , chemistry , biology , toxicity , organic chemistry
The intracellular level of cAMP increased by about 2-fold in CH3HgCl-treated neuroblastoma (NBP~) and glioma (C-6) cells in culture. The above change did not occur within a few ~ours of treatment, rather it is observed 3 days after treatment. CH3HgCI also reduced the PGEl-response in chronically treated glioma cells, but it failed to produce a similar effect in chronically treated NB cells. Dopamine and norepinephrine responses in NB cells and norepinephrine response in glioma cells were unaffected after chronic treatment of these cells by CH HgCI. In addition, 3 cAMP-dependent and independent phosphorylations of specific proteins were very sensitive to CH HgCI in both glioma and NB cells. The increases and decreases in the levels of p~osphorylation of proteins were observed after chronic treatment of cells with small concentrations (0.05-01 ~M) of CH HgCI. These data show that the cultures of NB and glioma cells may be useful in i~entifying the sensitive biological parameters that are affected by heavy metals.