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Inhibitory effects of methylmercuric chloride on high affinity 3 H‐digoxin binding in developing rat brain
Author(s) -
Chaturvedi A.K.,
Magill S.,
Brumleve S.J.,
Parmar S.S.
Publication year - 1983
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(83)90307-6
Subject(s) - pharmacy , state (computer science) , medicine , library science , family medicine , algorithm , computer science
Effects of methylmercuric chloride were investigated on the in vitro 3H-digoxin binding in crude synaptosomal preparations of brains obtained from developing rats. The high affinity binding of 3H-digoxin was found to increase with age. Methylmercuric chloride was found to decrease the binding and such a decrease was concentration dependent. The degree of in vitro inhibition by methylmercuric chloride (0.i mM) was found to be 69%, 80% and 76% in brain preparations obtained from 7, 8 and 14 days old rats, respectively. Mercuric chloride, used for comparative evaluation, was found to cause greater inhibition of 3H-digoxin binding. The percent degree of in vitro inhibition of 3H-digoxin binding observed with mercuric chloride (0.01 mM) was 26, 33 and 28 in brain preparations obtained from 7, 8 and 14 days old rats, respectively. These results have provided evidence that the greater sensitivity of methylmercuric chloride and mercuric chloride on 3H-digoxin binding in crude brain synaptosomal preparations obtained from 8 days old rats may presumably account for greater development of such specific 3H-digoxin receptors. (Supported in part by American Heart Association Dakota Affi l iate Inc., and the Max Baer Heart Fund.)