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Effect of astroglial growth factor on development of astrocytes
Author(s) -
Pettmann B.,
Labourdette G.,
Weibel M.,
Daune G.,
Sensenbrenner M.
Publication year - 1983
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(83)90288-5
Subject(s) - pascal (unit) , humanities , physics , art , quantum mechanics
The effects of glia maturation factor(GMF) on cell proliferation and differentiation were investigated with three astroglioma cells(GE-12, C6, and GA-I), oligodendroglioma cell(354A), and mixed glioma celI(LRM-55). The growth rates of all glioma cells were enhanced by GMF regardless of the presence or absence of serum, but the factor failed to make the saturation density surpass the control level observed in the medium without GMF even in the chemically defined medium(N2 medium). GMF markedly lowered the saturation density of oligodendroglioma cell in N2 medium. Although GMF increased the intracellular contents of S-100 protein 5-fold and CNPase activity 1.5-fold in oligodendroglioma cell, GMF conversely decreased the S-100 contents and GPDH activity in astroglioma cells. All the astroglioma cells secreted into the culture medium in large quantities a growth-promoting factor(s) which had the similar properties to that of GMF and stimulated the proliferation of normal glioblasts, but not oligodendroglioma cell. These findings imply that astroglioma cells are deprived of the differentiationpromoting response to GMF while oligodendroglioma cell preserves the response besides the proliferative response to GMF. (Supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Japan)