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Messenger RNA from human brain gliomas and meningiomas directs the synthesis of cholinesterase in microinjected Xenopus oocytes
Author(s) -
Razon N.,
ZevinSonkin D.,
Silman I.,
Soreq H.
Publication year - 1983
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(83)90259-9
Subject(s) - citation , library science , neuroscience , microbiology and biotechnology , psychology , biology , computer science
Messenger RNA from human brain gliomas and meningiomas directs the synthesis of cholinesterase in microinjected Xc~topcc5 oocytes. * " " ** " " ** If** Razon, N , •evln-Sonkln, D. , S11man, I. and Soreo~, . Weizmann Institute of Science, Dept. of Neurobiology**, Rehovot,and Ichilov ilospital, Dept. of Neurosurgery*, Tel-Aviv Medical Center, Israel. In order to investigate the biosynthesis of cholinesterases (Chl!s) it', particular eel] types within the human nervous system, we have examined the expression of acetylcholin esterase (ACHE) and of pseudocholinesterase (¢ChE) in frozen primary human glkogenous tumors and meningiomas. Brain ChE activity was 0.3-1.0 vmol of degraded 311-acetylcholine/min/gr tissue. Significant amounts (up to 40%) of "~ChE activity were found in most of the gliomas, whereas in all of the control tissues and in meningiomas AChE activity predominated (>90%). Sucrose gradient analysis revealed two AChE components, of sedimentation coefficient ca. 4.5S and 10S, in the neuroectoderm-originated gliomas and non-malignant brain tissue. In the mesenchyme-originated meningioma, the lighter (4.5S) form of AChE appears exclusively. So as to determine the level of Chll-mRNA, po]y(A)containing RNA from the above sources was microinjected into X~'~cl.x~5 oecytes. Oocvteproduced ChE, degrading 5 to 40 nmol ACh/~g mRNA/hr, was detected when mRNA from meningiomas and gliomas was injected. Our findings suggest that both dcdif'fercntiated gliogenous cells and meningeal cells within the human nervous system synthesize Chli, and t h a t the ChEs in t h e s e c e l l t y p e s d i f f e r in t h e i r s u b s t r a t e s p e c i f i c i t i e s and m i g r a t i o n p r o p e r t i e s . Suppor ted by the USARMED and the Muscu la r Dys t rophy A s s o c i a t i o n .