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Workshop: New methods in developmental neurobiology. “Recombinant DNA and cDNA probes”
Author(s) -
Mallet J.,
Privat A.
Publication year - 1983
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(83)90253-8
Subject(s) - citation , mallet , cdna library , computer science , recombinant dna , associate editor , library science , cognitive science , complementary dna , information retrieval , genetics , biology , psychology , gene , history , archaeology
The purpose of this workshop is to acquaint participants with the most common DNA recombinant techniques and the attendant vocabulary. Several strategies can be employed to isolate a specific gene. From the various exemples which will be discussed it will become clear that the cloning of a gene can be accomplished from very little tissue, even if it is not represented in relative abundance in mRNA populations. This point is important in considering that the brain is a very heterogeneous organ. Furthermore, it will also become appearant that the identification of a given gene might be easier than the isolation of the corresponding protein. This new trend should have a great impact in neurobiology. These techniques might also provide a way of uncovering new substances (transmittots, hormones, receptors) present in too small amounts to be pinpointed by classical biochemical techniques. More particularly, complementary cDNAs which are characteristic of a given tissue or of a given stage in development should be obtained rather easily and this approach may be complementary to the search for monospecific antibodies.