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Axonal transport of gangliosides in the visual system of the developing chick embryo
Author(s) -
Panzetta P.,
Chiarenza A.P.,
Maccioni H.J.F.
Publication year - 1983
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(83)90041-2
Subject(s) - embryo , ganglioside , chemistry , axoplasmic transport , optic tectum , tectum , anatomy , andrology , biology , biochemistry , endocrinology , central nervous system , microbiology and biotechnology , medicine , midbrain
Chick embryos aged 7–18 days of development were injected with N ‐[ 3 H]acetylmannosamine into one eye. The labeling of gangliosides was significantly higher in the contralateral‐ than in the ipsilateral‐optic tectum in embryos aged 14 days or older. Comparison of the radioactivities of thin‐layer chromatograms of gangliosides from the contralateral‐ and ipsilateral‐optic tecta showed that in the 14‐day‐old embryo about 56 and 29% of the transported radioactivity chromatographed as GD1a and GT1, respectively. Most of the remaining radioactivity chromatographed in the zone of less complex gangliosides (GM2‐GD3, 3%; GM3, 6%). In the 18‐day‐old embryo about 72% of the transported radioactivity chromatographed as GD1a; the radioactivity in the zone of GT1 was reduced to about 2% and that in the less complex gangliosides remained low (about 14% in the zone of GM2‐GD3 and 7% in the zone of GM3).