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A comparison of the regional ontogenesis of nicotine‐ and muscarine‐like binding sites in mouse brain
Author(s) -
Falkeborn Y.,
Larsson C.,
Nordberg A.,
Slanina P.
Publication year - 1983
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(83)90031-x
Subject(s) - muscarine , nicotine , hippocampus , medicine , endocrinology , choline acetyltransferase , quinuclidinyl benzilate , muscarinic acetylcholine receptor , cerebellum , ligand (biochemistry) , chemistry , cholinergic , receptor , cortex (anatomy) , midbrain , hippocampal formation , central nervous system , biology , neuroscience , biochemistry
The postnatal development of the cholinergic neurotransmitter system was studied in the cortex, hippocampus, midbrain and cerebellum of 3‐, 7‐, 12‐, 17‐ and 30‐day‐old NMRI mice. The concentration of muscarine‐like binding sites determined with [ 3 H]quinuclidinyl benzilate as a ligand increased progressively with age. A similar developmental pattern was found for the activity of a presynaptic marker, choline acetyltransferase (ChAT). When expressed as a percentage of the values for 30‐day‐old mice, however, the muscarine‐like receptors were parallel but preceding the development of ChAT in all the brain regions studied. The concentration of nicotine‐like binding sites studied with [ 3 H]α‐bungaro‐toxin as a ligand gradually increased with age in the cortex, hippocampus and midbrain, with a peak between days 7 and 12, followed by a decrease towards day 30. With [ 3 H]tubocurarine as a ligand, on the other hand, the concentration of nicotine‐like binding sites was in general high at 3 days and gradually decreased with age, suggesting that different subpopulations of nicotine‐like receptors might be determined when the two ligands are used.