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Conditioned medium from spinal cord cells stimulates DRG cells to migrate, cluster and rearrange in dissociated cell cultures
Author(s) -
Klotz Kathleen L.,
Neale Joseph H.
Publication year - 1983
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(83)90027-8
Subject(s) - spinal cord , mitosis , microbiology and biotechnology , cleavage (geology) , sensory system , cell culture , embryo , biology , cell , neuroscience , chemistry , anatomy , genetics , paleontology , fracture (geology)
Murine spinal sensory neurons migrate in dissociated cell culture resulting in convergence, divergence and cluster rearrangement by the cells. We found that culture medium which had been conditioned by the growth of spinal cord cells stimulated the convergence of DRG cells into clusters. Binuclear sensory neurons, which are often found in these cultures, failed to migrate or proceed with mitotic cleavage during the 2 weeks of observation. We conclude that these cells were arrested in mitosis during or shortly after their removal from the 13‐day‐old embryo.